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Promega goscript tm reverse transcriptase kit
Goscript Tm Reverse Transcriptase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscript+tm+reverse+transcriptase+kit/goscript+reverse+transcriptase+kit/bio_rxiv__2025__05__21__655246-222-13-18
Average 90 stars, based on 1 article reviews
goscript tm reverse transcriptase kit - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Random Hexamer:

Article Title: Cancer-derived mutation in the OGA stalk domain promotes cell malignancy through dysregulating PDLIM7 and p53
Article Snippet: After 48 h of doxycycline induction, RNA purification was performed using TRIzol reagent (Invitrogen). .. 1.5 μg RNA with Oligo(dT) 18 and random hexamer primers were used for cDNA synthesis. cDNA synthesis was conducted by Maxima H Minus First Strand cDNA Synthesis Kit with DNase (Life Technology) or GoScript TM Reverse Transcriptase kit (Promega) according to manufacturer’s instructions. cDNA samples were stored at −80 °C and used for real-time qPCR analysis within one week. .. The PCR sample was prepared using 2 ng cDNA and PowerUp SYBR Green Master Mix (Life Technology) following manufacturer’s instruction.

cDNA Synthesis:

Article Title: Cancer-derived mutation in the OGA stalk domain promotes cell malignancy through dysregulating PDLIM7 and p53
Article Snippet: After 48 h of doxycycline induction, RNA purification was performed using TRIzol reagent (Invitrogen). .. 1.5 μg RNA with Oligo(dT) 18 and random hexamer primers were used for cDNA synthesis. cDNA synthesis was conducted by Maxima H Minus First Strand cDNA Synthesis Kit with DNase (Life Technology) or GoScript TM Reverse Transcriptase kit (Promega) according to manufacturer’s instructions. cDNA samples were stored at −80 °C and used for real-time qPCR analysis within one week. .. The PCR sample was prepared using 2 ng cDNA and PowerUp SYBR Green Master Mix (Life Technology) following manufacturer’s instruction.

Article Title: Hypoxia signaling in the equine small intestine: Expression and distribution of hypoxia inducible factors during experimental ischemia
Article Snippet: The RNA concentration and quality were determined with the aid of a spectrophotometer (BioPhotometer, Eppendorf, Wesseling-Berzdorf, Germany ). .. In total, 1 μg of high-quality RNA was used for cDNA synthesis using the GoScript TM Reverse Transcriptase Kit ( Promega ) according to the manufacturer's instructions with a MJ Research PTC-200 Peltier Thermal Cycler ( Bio-Rad, Feldkirchen, Germany ). .. For qPCR, the resulting cDNA was diluted 1:20 and 2 μl were used in a 20 μl reaction volume containing 10 μl of a ready-to-use premix of SYBR Green I dye, dNTPs, stabilizers and enhancers (GoTaq ® , Promega ), 112 nM primer mix, and DNase-free water.

Reverse Transcription:

Article Title: Cancer-derived mutation in the OGA stalk domain promotes cell malignancy through dysregulating PDLIM7 and p53
Article Snippet: After 48 h of doxycycline induction, RNA purification was performed using TRIzol reagent (Invitrogen). .. 1.5 μg RNA with Oligo(dT) 18 and random hexamer primers were used for cDNA synthesis. cDNA synthesis was conducted by Maxima H Minus First Strand cDNA Synthesis Kit with DNase (Life Technology) or GoScript TM Reverse Transcriptase kit (Promega) according to manufacturer’s instructions. cDNA samples were stored at −80 °C and used for real-time qPCR analysis within one week. .. The PCR sample was prepared using 2 ng cDNA and PowerUp SYBR Green Master Mix (Life Technology) following manufacturer’s instruction.

Article Title: Hypoxia signaling in the equine small intestine: Expression and distribution of hypoxia inducible factors during experimental ischemia
Article Snippet: The RNA concentration and quality were determined with the aid of a spectrophotometer (BioPhotometer, Eppendorf, Wesseling-Berzdorf, Germany ). .. In total, 1 μg of high-quality RNA was used for cDNA synthesis using the GoScript TM Reverse Transcriptase Kit ( Promega ) according to the manufacturer's instructions with a MJ Research PTC-200 Peltier Thermal Cycler ( Bio-Rad, Feldkirchen, Germany ). .. For qPCR, the resulting cDNA was diluted 1:20 and 2 μl were used in a 20 μl reaction volume containing 10 μl of a ready-to-use premix of SYBR Green I dye, dNTPs, stabilizers and enhancers (GoTaq ® , Promega ), 112 nM primer mix, and DNase-free water.

Article Title: SARS-CoV-2 ORF3a drives dynamic dense body formation for optimal viral infectivity
Article Snippet: Strand-specific RNA-SEQ libraries were prepared using a TruSeq-based mRNA RNA-SEQ library protocol (Illumina). .. For Particle-to-PFU ratio measurement, RNA was extracted from virus stocks using NucleoSpin 96 RNA (Macherey-Nagel, 740709), before reverse transcription to cDNA using the GoScript TM Reverse Transcriptase kit (Promega, A5001) following the manufacturer’s instructions. qPCR was performed using the PowerUp TM SYBR TM Green Master Mix kit (Thermo Fisher Scientific, A25742) with 0.1 μL of cDNA as a template on an Applied Biosystems StepOnePlus TM Real-Time PCR system (Thermo Fisher Scientific). .. Library quality and quantity were assessed using the Agilent bio-analyzer, and libraries were sequenced using an Illumina NovaSEQ6000 (Illumina provided reagents and protocols).

Article Title: Multiple-omics analyses of the Alviniconcha holobiont reveal multi-faceted adaptations to deep-sea hydrothermal vents
Article Snippet: According to the manufacturer’s instructions, total RNA was extracted using an Eastep ® Super Total RNA Extraction Kit (Promega, USA) and purified using the RNA purification and recovery Kit (Genstone, China). .. The first strand of cDNA from the gill RNA was synthesized using a GoScript TM Reverse Transcriptase kit (Promega, USA) according to the manufacturer’s instruction and used for PCR amplification (These primers were shown in Table S17 in Supplementary Table). .. Then, the PCR products were cloned into the pEASY-T3 vector (TransGen, China), and the clone plasmids were purified using the TIANprep Mini Plasmid Kit (TIANGEN, China) and sequenced using the Sanger method.

Article Title: Convergent evolution of noxious heat sensing by TRPA5, a novel class of heat sensor in Rhodnius prolixus
Article Snippet: Antennae from twenty Rhodnius adult individuals were obtained from a laboratory culture (Orchard lab, University of Toronto Mississauga, Canada) and stored in DNA/RNA Shield TM reagent (Zymo Research). .. Tissues were disrupted in Trizol using a Premium Multi-Gen 7XL Homogenizer (PRO Scientific) and RNA was subsequently extracted using the Direct-zol RNA kit (Zymo Research), including a DNAse step to remove genomic DNA contamination. cDNA was synthesized from 1ug Total RNA using the GoScript TM Reverse Transcriptase kit (Promega) and random hexamers following the recommended manufacturers’ protocol. .. RNA and cDNA qualities were verified using a Nanodrop (Nanodrop 2000/2000c UV-vis spectrophotometer, Thermo Scientific) and quantified using a Qubit Fluorometer (ThermoFisher).

Article Title: The NtSPL Gene Family in Nicotiana tabacum : Genome-Wide Investigation and Expression Analysis in Response to Cadmium Stress
Article Snippet: .. RNA was extracted from the above plant material according to the instructions of the RNAsimple Total RNA Extraction Kit (Catalog No. DP419 and DP424) from Tiangen, Beijing, China. cDNA was synthesized from the first strand of the plant samples according to the GoScript TM Reverse Transcriptase Kit from Promega, Madison, USA, and the synthesized cDNA was used for subsequent qPCR analysis. .. Three tobacco seedlings were used in each group for RNA extraction experiments. qPCR was performed on the CFX96 TM real-time fluorescence quantification platform using SYBR green luciferase (Novoprotein, Suzhou, China) with a reaction program of 45 cycles of 95 °C for 1 min; 95 °C for 15 s; and 60 °C for 30 s. The qPCR primers for the Nta-miR156 and NtSPL gene family were designed by Primer Premier 6.0 ( ).

Article Title: Comparative Transcriptome Analysis Reveals the Effect of miR156a Overexpression on Mineral Nutrient Homeostasis in Nicotiana tabacum
Article Snippet: .. Subsequently, 5 μg of total RNA was reverse transcribed and synthesized into cDNA using the GoScript TM Reverse Transcriptase Kit (Promega, Madison, WI, USA). ..

Article Title: Dimethyl Sulfoxide (DMSO) as a Potential Source of Interference in Research Related to Sulfur Metabolism—A Preliminary Study
Article Snippet: .. The reverse transcription of the total RNA from the cell samples was performed using a GoScript TM Reverse Transcriptase Kit according to the manufacturer’s protocol (Promega, Madison, WI, USA). .. For the reaction, 2 μg of the total RNA were combined with water pretreated with diethylpyrocarbonate (DEPC-H 2 O, Thermo Fisher Scientific, Waltham, MA, USA) and with 1 μL of Oligo(dT) 15 primer (0.5 μg/reaction, Thermo Fisher Scientific, Waltham, MA, USA) and incubated for 5 min at 70 °C.

Real-time Polymerase Chain Reaction:

Article Title: Cancer-derived mutation in the OGA stalk domain promotes cell malignancy through dysregulating PDLIM7 and p53
Article Snippet: After 48 h of doxycycline induction, RNA purification was performed using TRIzol reagent (Invitrogen). .. 1.5 μg RNA with Oligo(dT) 18 and random hexamer primers were used for cDNA synthesis. cDNA synthesis was conducted by Maxima H Minus First Strand cDNA Synthesis Kit with DNase (Life Technology) or GoScript TM Reverse Transcriptase kit (Promega) according to manufacturer’s instructions. cDNA samples were stored at −80 °C and used for real-time qPCR analysis within one week. .. The PCR sample was prepared using 2 ng cDNA and PowerUp SYBR Green Master Mix (Life Technology) following manufacturer’s instruction.

Article Title: SARS-CoV-2 ORF3a drives dynamic dense body formation for optimal viral infectivity
Article Snippet: Strand-specific RNA-SEQ libraries were prepared using a TruSeq-based mRNA RNA-SEQ library protocol (Illumina). .. For Particle-to-PFU ratio measurement, RNA was extracted from virus stocks using NucleoSpin 96 RNA (Macherey-Nagel, 740709), before reverse transcription to cDNA using the GoScript TM Reverse Transcriptase kit (Promega, A5001) following the manufacturer’s instructions. qPCR was performed using the PowerUp TM SYBR TM Green Master Mix kit (Thermo Fisher Scientific, A25742) with 0.1 μL of cDNA as a template on an Applied Biosystems StepOnePlus TM Real-Time PCR system (Thermo Fisher Scientific). .. Library quality and quantity were assessed using the Agilent bio-analyzer, and libraries were sequenced using an Illumina NovaSEQ6000 (Illumina provided reagents and protocols).

Article Title: The NtSPL Gene Family in Nicotiana tabacum : Genome-Wide Investigation and Expression Analysis in Response to Cadmium Stress
Article Snippet: .. RNA was extracted from the above plant material according to the instructions of the RNAsimple Total RNA Extraction Kit (Catalog No. DP419 and DP424) from Tiangen, Beijing, China. cDNA was synthesized from the first strand of the plant samples according to the GoScript TM Reverse Transcriptase Kit from Promega, Madison, USA, and the synthesized cDNA was used for subsequent qPCR analysis. .. Three tobacco seedlings were used in each group for RNA extraction experiments. qPCR was performed on the CFX96 TM real-time fluorescence quantification platform using SYBR green luciferase (Novoprotein, Suzhou, China) with a reaction program of 45 cycles of 95 °C for 1 min; 95 °C for 15 s; and 60 °C for 30 s. The qPCR primers for the Nta-miR156 and NtSPL gene family were designed by Primer Premier 6.0 ( ).

Virus:

Article Title: SARS-CoV-2 ORF3a drives dynamic dense body formation for optimal viral infectivity
Article Snippet: Strand-specific RNA-SEQ libraries were prepared using a TruSeq-based mRNA RNA-SEQ library protocol (Illumina). .. For Particle-to-PFU ratio measurement, RNA was extracted from virus stocks using NucleoSpin 96 RNA (Macherey-Nagel, 740709), before reverse transcription to cDNA using the GoScript TM Reverse Transcriptase kit (Promega, A5001) following the manufacturer’s instructions. qPCR was performed using the PowerUp TM SYBR TM Green Master Mix kit (Thermo Fisher Scientific, A25742) with 0.1 μL of cDNA as a template on an Applied Biosystems StepOnePlus TM Real-Time PCR system (Thermo Fisher Scientific). .. Library quality and quantity were assessed using the Agilent bio-analyzer, and libraries were sequenced using an Illumina NovaSEQ6000 (Illumina provided reagents and protocols).

Synthesized:

Article Title: Multiple-omics analyses of the Alviniconcha holobiont reveal multi-faceted adaptations to deep-sea hydrothermal vents
Article Snippet: According to the manufacturer’s instructions, total RNA was extracted using an Eastep ® Super Total RNA Extraction Kit (Promega, USA) and purified using the RNA purification and recovery Kit (Genstone, China). .. The first strand of cDNA from the gill RNA was synthesized using a GoScript TM Reverse Transcriptase kit (Promega, USA) according to the manufacturer’s instruction and used for PCR amplification (These primers were shown in Table S17 in Supplementary Table). .. Then, the PCR products were cloned into the pEASY-T3 vector (TransGen, China), and the clone plasmids were purified using the TIANprep Mini Plasmid Kit (TIANGEN, China) and sequenced using the Sanger method.

Article Title: Convergent evolution of noxious heat sensing by TRPA5, a novel class of heat sensor in Rhodnius prolixus
Article Snippet: Antennae from twenty Rhodnius adult individuals were obtained from a laboratory culture (Orchard lab, University of Toronto Mississauga, Canada) and stored in DNA/RNA Shield TM reagent (Zymo Research). .. Tissues were disrupted in Trizol using a Premium Multi-Gen 7XL Homogenizer (PRO Scientific) and RNA was subsequently extracted using the Direct-zol RNA kit (Zymo Research), including a DNAse step to remove genomic DNA contamination. cDNA was synthesized from 1ug Total RNA using the GoScript TM Reverse Transcriptase kit (Promega) and random hexamers following the recommended manufacturers’ protocol. .. RNA and cDNA qualities were verified using a Nanodrop (Nanodrop 2000/2000c UV-vis spectrophotometer, Thermo Scientific) and quantified using a Qubit Fluorometer (ThermoFisher).

Article Title: The NtSPL Gene Family in Nicotiana tabacum : Genome-Wide Investigation and Expression Analysis in Response to Cadmium Stress
Article Snippet: .. RNA was extracted from the above plant material according to the instructions of the RNAsimple Total RNA Extraction Kit (Catalog No. DP419 and DP424) from Tiangen, Beijing, China. cDNA was synthesized from the first strand of the plant samples according to the GoScript TM Reverse Transcriptase Kit from Promega, Madison, USA, and the synthesized cDNA was used for subsequent qPCR analysis. .. Three tobacco seedlings were used in each group for RNA extraction experiments. qPCR was performed on the CFX96 TM real-time fluorescence quantification platform using SYBR green luciferase (Novoprotein, Suzhou, China) with a reaction program of 45 cycles of 95 °C for 1 min; 95 °C for 15 s; and 60 °C for 30 s. The qPCR primers for the Nta-miR156 and NtSPL gene family were designed by Primer Premier 6.0 ( ).

Article Title: Comparative Transcriptome Analysis Reveals the Effect of miR156a Overexpression on Mineral Nutrient Homeostasis in Nicotiana tabacum
Article Snippet: .. Subsequently, 5 μg of total RNA was reverse transcribed and synthesized into cDNA using the GoScript TM Reverse Transcriptase Kit (Promega, Madison, WI, USA). ..

Polymerase Chain Reaction:

Article Title: Multiple-omics analyses of the Alviniconcha holobiont reveal multi-faceted adaptations to deep-sea hydrothermal vents
Article Snippet: According to the manufacturer’s instructions, total RNA was extracted using an Eastep ® Super Total RNA Extraction Kit (Promega, USA) and purified using the RNA purification and recovery Kit (Genstone, China). .. The first strand of cDNA from the gill RNA was synthesized using a GoScript TM Reverse Transcriptase kit (Promega, USA) according to the manufacturer’s instruction and used for PCR amplification (These primers were shown in Table S17 in Supplementary Table). .. Then, the PCR products were cloned into the pEASY-T3 vector (TransGen, China), and the clone plasmids were purified using the TIANprep Mini Plasmid Kit (TIANGEN, China) and sequenced using the Sanger method.

Amplification:

Article Title: Multiple-omics analyses of the Alviniconcha holobiont reveal multi-faceted adaptations to deep-sea hydrothermal vents
Article Snippet: According to the manufacturer’s instructions, total RNA was extracted using an Eastep ® Super Total RNA Extraction Kit (Promega, USA) and purified using the RNA purification and recovery Kit (Genstone, China). .. The first strand of cDNA from the gill RNA was synthesized using a GoScript TM Reverse Transcriptase kit (Promega, USA) according to the manufacturer’s instruction and used for PCR amplification (These primers were shown in Table S17 in Supplementary Table). .. Then, the PCR products were cloned into the pEASY-T3 vector (TransGen, China), and the clone plasmids were purified using the TIANprep Mini Plasmid Kit (TIANGEN, China) and sequenced using the Sanger method.

RNA Extraction:

Article Title: The NtSPL Gene Family in Nicotiana tabacum : Genome-Wide Investigation and Expression Analysis in Response to Cadmium Stress
Article Snippet: .. RNA was extracted from the above plant material according to the instructions of the RNAsimple Total RNA Extraction Kit (Catalog No. DP419 and DP424) from Tiangen, Beijing, China. cDNA was synthesized from the first strand of the plant samples according to the GoScript TM Reverse Transcriptase Kit from Promega, Madison, USA, and the synthesized cDNA was used for subsequent qPCR analysis. .. Three tobacco seedlings were used in each group for RNA extraction experiments. qPCR was performed on the CFX96 TM real-time fluorescence quantification platform using SYBR green luciferase (Novoprotein, Suzhou, China) with a reaction program of 45 cycles of 95 °C for 1 min; 95 °C for 15 s; and 60 °C for 30 s. The qPCR primers for the Nta-miR156 and NtSPL gene family were designed by Primer Premier 6.0 ( ).



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